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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FRY Polyclonal antibody (32075-1-AP)

    WB analysis using 32075-1-AP (same clone as 32075-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 32075-1-AP (FRY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FRY Polyclonal antibody (32075-1-AP)

    IHC staining of human pancreas cancer using 32075-1-AP (same clone as 32075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FRY Polyclonal antibody (32075-1-AP)

    IHC staining of human pancreas cancer using 32075-1-AP (same clone as 32075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using FRY Polyclonal antibody (32075-1-AP)

    IHC staining of human urothelial carcinoma using 32075-1-AP (same clone as 32075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 32075-1-AP (FRY antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using FRY Polyclonal antibody (32075-1-AP)

    IF Staining of THP-1 using 32075-1-AP (same clone as 32075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FRY antibody (32075-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32075-1-PBS in a different storage buffer formulation.

FRY Polyclonal antibody, PBS Only

FRY Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32075-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C13orf14, FRY Microtubule Binding Protein, Protein furry homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32075-1-PBS targets FRY in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34959

Product name: Recombinant human FRY protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2534-2680 aa of NM_023037

Sequence: LEHSDLIMTLSPSEETNPMELLTTACDSTPAEPHSFNTRMSSFDASLPDMNNLQISEGSKAEAVREEEDTTVHEDDLSSSINELPAAFECSDSFSLDMTEGEEKGNRALDQFTLASFGEGDRGVSPPPSPFFSAILAAFQPAACDDA

相同性解析による交差性が予測される生物種
Full Name furry homolog (Drosophila)
Calculated molecular weight339 kDa
Observed molecular weight340 kDa
GenBank accession numberNM_023037
Gene Symbol FRY
Gene ID (NCBI) 10129
RRIDAB_3742522
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5TBA9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The FRY gene encodes a protein involved in cell cycle regulation and DNA damage response. It plays a crucial role in the G2/M checkpoint, ensuring cells complete DNA repair before entering mitosis by activating the ATR-CHK1 signaling pathway. FRY is expressed in various tissues, including brain, liver, and testis, with notable expression in Sertoli and spermatogonial cells. Aberrant FRY function is associated with multiple diseases, such as cancer and neurodegenerative disorders. In cancer cells, FRY expression can influence proliferation and survival. Understanding FRY's role in cell cycle control and genome stability is vital for elucidating disease mechanisms and developing targeted therapies.

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