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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FRMD1 Polyclonal antibody (31908-1-AP)

    WB analysis using 31908-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31908-1-AP (FRMD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FRMD1 Polyclonal antibody (31908-1-AP)

    WB analysis of K-562 using 31908-1-AP

    WB result of FRMD1 antibody (31908-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FRMD1 transfected K-562 cells.

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  • KD/KO Validated

FRMD1 Polyclonal antibody

FRMD1 Polyclonal Antibody for WB, ELISA
Cat No. 31908-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FERM domain-containing protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, K-562 cells, mouse testis tissue, mouse thymus tissue, rat thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31908-1-AP targets FRMD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FRMD1 fusion protein Ag36382 相同性解析による交差性が予測される生物種
Full Name FERM domain containing 1
Observed molecular weight70 kDa
GenBank accession numberBC137144
Gene Symbol FRMD1
Gene ID (NCBI) 79981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8N878
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The FERM structural domain of FRMD1 (FERM Domain Containing 1) is widely found in cytoskeleton-associated proteins, which are capable of connecting the cytoskeleton to the cell membrane and is involved in signaling. FRMD1 is predicted to be involved in the positive regulation of the Hippo signaling pathway and may play a role in the cytoplasmic side of the cytoskeleton and apical plasma membrane. Although there are fewer studies on the role of FRMD1 in disease, its expression pattern in cancer and lymphoid tissues suggests that it may have important biological functions.

Protocols

Product Specific Protocols
WB protocol for FRMD1 antibody 31908-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols