FRK Polyclonal antibody
FRK Polyclonal Antibody for WB, IF, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 16197-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, K-562 cells, NIH/3T3 cells |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 16197-1-AP is FRK Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FRK fusion protein Ag10372 相同性解析による交差性が予測される生物種 |
Full Name | fyn-related kinase |
Calculated molecular weight | 505 aa, 56 kDa |
Observed molecular weight | 54-57 kDa |
GenBank accession number | BC012916 |
Gene symbol | FRK |
Gene ID (NCBI) | 2444 |
RRID | AB_10639525 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The fyn-related kinase (FRK) belongs to the tyrosine kinase family of protein kinases and is also named as PTK5(protein-tyrosine kinase 5), RAK(Nuclear tyrosine protein kinase). The Frk is a tyrosine kinase expressed in various tissues, such as the gastrointestinal tract and pancreatic islet cells. It affects pancreatic beta cell number during embryogenesis and promotes beta cell cytotoxic signals in response to streptozotocin(PMID:20157399). It may function as a tumor suppressor(PMID:19345329).
Protocols
Product Specific Protocols | |
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WB protocol for FRK antibody 16197-1-AP | Download protocol |
IHC protocol for FRK antibody 16197-1-AP | Download protocol |
IF protocol for FRK antibody 16197-1-AP | Download protocol |
FC protocol for FRK antibody 16197-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |