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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using FREM1 Polyclonal antibody (13086-1-AP)

    WB analysis of human plasma using 13086-1-AP (same clone as 13086-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 13086-1-AP (FREM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FREM1 Polyclonal antibody (13086-1-AP)

    WB analysis of HepG2 using 13086-1-AP (same clone as 13086-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13086-1-AP (FREM1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocellular cancer using FREM1 Polyclonal antibody (13086-1-AP)

    IHC staining of human hepatocellular cancer using 13086-1-AP (same clone as 13086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 13086-1-AP (FREM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FREM1 Polyclonal antibody (13086-1-AP)

    IHC staining of human breast cancer using 13086-1-AP (same clone as 13086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FREM1 Polyclonal antibody (13086-1-AP)

    IHC staining of human breast cancer using 13086-1-AP (same clone as 13086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13086-1-AP (FREM1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FREM1 Polyclonal antibody (13086-1-AP)

    IF Staining of HepG2 using 13086-1-AP (same clone as 13086-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FREM1 antibody (13086-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13086-1-PBS in a different storage buffer formulation.

FREM1 Polyclonal antibody, PBS Only

FREM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13086-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C9orf143, C9orf145, C9orf154, FRAS1-related extracellular matrix protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13086-1-PBS targets FREM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3617

Product name: Recombinant human FREM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 412-710 aa of BC031064

Sequence: SSSTTSGSFHLERRPLPSSMQLAVIRGDTLRGFDSTDLSQRKLRTRGNGKTVRPSSVYRNGTDIIYNYHGIVSLKLEDDSFPTHKRKAKVSIISQPQKTIKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELKGLFHFEEGIQKLYQCNGIAWKAWSPQTKDVEDKSCPAGWHQHSGYCHILITEQKGTWNAAAQACREQYLGNLVTVFSRQHMRWLWDIGGRKSFWIGLNDQVHAGHWEWIGGEPVAFTNGRRGPSPRSKLGKSCVLVQRQGKWQTKDCRRAKPHNYGCSRKL

相同性解析による交差性が予測される生物種
Full Name FRAS1 related extracellular matrix 1
Calculated molecular weight2179aa, 244 kDa, 149.3 kDa
Observed molecular weight 40-43 kDa, 70-80 kDa
GenBank accession numberBC031064
Gene Symbol FREM1
Gene ID (NCBI) 158326
RRIDAB_2877915
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5H8C1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FREM1, C9orf143, C9orf145, C9orf154 and QBRICK, is an extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. This antibody recognizes isoform 1 (244 kDa), isform 2 (80 kDa) and isoform 3 (43 kDa).

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