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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using FOXO6 Polyclonal antibody (19122-1-AP)

    WB analysis of mouse brain using 19122-1-AP (same clone as 19122-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOXO6 Polyclonal antibody (19122-1-AP)

    WB analysis using 19122-1-AP (same clone as 19122-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using FOXO6 Polyclonal antibody (19122-1-AP)

    IP experiment of mouse brain using 19122-1-AP (same clone as 19122-1-PBS)

    IP result of anti-FOXO6 (IP:19122-1-AP, 3ug; Detection:19122-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FOXO6 Polyclonal antibody (19122-1-AP)

    IHC staining of mouse brain using 19122-1-AP (same clone as 19122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FOXO6 Polyclonal antibody (19122-1-AP)

    IHC staining of mouse brain using 19122-1-AP (same clone as 19122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using FOXO6 Polyclonal antibody (19122-1-AP)

    IHC staining of human brain using 19122-1-AP (same clone as 19122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using FOXO6 Polyclonal antibody (19122-1-AP)

    IHC staining of human brain using 19122-1-AP (same clone as 19122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using FOXO6 Polyclonal antibody (19122-1-AP)

    IHC staining of human gliomas using 19122-1-AP (same clone as 19122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using FOXO6 Polyclonal antibody (19122-1-AP)

    IHC staining of human gliomas using 19122-1-AP (same clone as 19122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FOXO6 Polyclonal antibody (19122-1-AP)

    IF Staining of A549 using 19122-1-AP (same clone as 19122-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FOXO6 antibody (19122-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FOXO6 Polyclonal antibody (19122-1-AP)

    IF Staining of A549 using 19122-1-AP (same clone as 19122-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FOXO6 antibody (19122-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19122-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FOXO6 Polyclonal antibody, PBS Only

FOXO6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19122-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19122-1-PBS targets FOXO6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name forkhead box protein O6
Calculated molecular weight 51 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberAI361654
Gene Symbol FOXO6
Gene ID (NCBI) 100132074
RRIDAB_10597247
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA8MYZ6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system. FOXO6 has an isoform of 70 kDa when modified after translation (PMID:23639108).

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