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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXJ2 Polyclonal antibody (28301-1-AP)

    WB analysis using 28301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28301-1-AP (FOXJ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using FOXJ2 Polyclonal antibody (28301-1-AP)

    WB analysis of HuH-7 using 28301-1-AP

    WB result of FOXJ2 antibody (28301-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXJ2 transfected HuH-7 cells.

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  • KD/KO Validated

FOXJ2 Polyclonal antibody

FOXJ2 Polyclonal Antibody for WB, ELISA
Cat No. 28301-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FHX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHuH-7 cells, U-87 MG cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28301-1-AP targets FOXJ2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXJ2 fusion protein Ag27345 相同性解析による交差性が予測される生物種
Full Name forkhead box J2
Calculated molecular weight 574 aa, 62 kDa
Observed molecular weight70 kDa
GenBank accession numberBC136305
Gene Symbol FOXJ2
Gene ID (NCBI) 55810
RRIDAB_3669651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0K8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fork head box J2 (FOXJ2) is a transcription factor discovered in mammals and other vertebrates in 2000, which belongs to the Fork head box (Fox) transcription factor family, which shares a conserved DNA-binding domain, known as Fork Head. FOXJ2 participates in the regulation of cell proliferation, differentiation, and migration by targeting downstream genes through its DNA binding domain, and plays fundamental roles in embryonic development, tumorigenesis, and the progression of certain cancers. The expression patterns of FOXJ2 have been reported to be aberrant in a variety of cancers, including breast cancer, extra-hepatic cholangiocarcinoma, nasopharyngeal carcinoma, glioma, and non-small cell lung cancer (PMID: 28769576, PMID: 33725831, PMID: 35908066). This antibody has been validated for knockdown.

Protocols

Product Specific Protocols
WB protocol for FOXJ2 antibody 28301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols