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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXJ1 Polyclonal antibody (25903-1-AP)

    WB analysis using 25903-1-AP (same clone as 25903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25903-1-AP (FOXJ1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25903-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FOXJ1 Polyclonal antibody (25903-1-AP)

    IHC staining of mouse liver using 25903-1-AP (same clone as 25903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25903-1-AP (FOXJ1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25903-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25903-1-PBS targets FOXJ1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXJ1 fusion protein Ag20827 相同性解析による交差性が予測される生物種
Full Name forkhead box J1
Calculated molecular weight 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC046460
Gene Symbol FOXJ1
Gene ID (NCBI) 2302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92949
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FOXJ1 (Forkhead box protein J1) acts by activating the transcription of genes mediating motile cilia's assembly, such as CFAP157. It binds the DNA consensus sequences 5'-HWDTGTTTGTTTA-3' or 5'-KTTTGTTGTTKTW-3' (where H is not G, W is A or T, D is not C, and K is G or T), and activates the transcription of a variety of ciliary proteins in the developing brain and lung (PMID: 31630787).