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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FOXE3 Polyclonal antibody (55301-1-AP)

    WB analysis of HepG2 using 55301-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using FOXE3 Polyclonal antibody (55301-1-AP)

    WB analysis of A375 using 55301-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using FOXE3 Polyclonal antibody (55301-1-AP)

    WB analysis of A375 using 55301-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using FOXE3 Polyclonal antibody (55301-1-AP)

    WB analysis of L02 using 55301-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FOXE3 Polyclonal antibody (55301-1-AP)

    WB analysis of HepG2 using 55301-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

FOXE3 Polyclonal antibody

FOXE3 Polyclonal Antibody for WB, ELISA
Cat No. 55301-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ASMD, FKHL12, forkhead box E3, Forkhead box protein E3, FOXE3, FREAC 8, FREAC8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHepG2 cells, A375 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55301-1-AP targets FOXE3 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name forkhead box E3
Calculated molecular weight 33 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberNM_012186
Gene Symbol FOXE3
Gene ID (NCBI) 2301
RRIDAB_10896313
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13461
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXE3, also named as FKHL12 and FREAC8, is a forkhead transcription factor expressed in the lens which is located at chromosome 1p32, have been associated with both recessive and dominant ocular disease. Defects in FOXE3 are a cause of anterior segment mesenchymal dysgenesis (ASMD). Defects in FOXE3 are a cause of congenital primary aphakia (CPA). This antibody is specific to FOXE3.

Protocols

Product Specific Protocols
WB protocol for FOXE3 antibody 55301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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