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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using FOXD4L6 Polyclonal antibody (22081-1-AP)

    WB analysis of A431 using 22081-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22081-1-AP (FOXD4L6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FOXD4L6 Polyclonal antibody (22081-1-AP)

    WB analysis of HepG2 using 22081-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22081-1-AP (FOXD4L6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FOXD4L6 Polyclonal antibody (22081-1-AP)

    WB analysis of Jurkat using 22081-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22081-1-AP (FOXD4L6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

FOXD4L6 Polyclonal antibody

FOXD4L6 Polyclonal Antibody for WB, ELISA
Cat No. 22081-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

forkhead box D4 like 6, Forkhead box protein D4 like 6, FOXD4 like 6, FOXD4L6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22081-1-AP targets FOXD4L6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17247

Product name: Recombinant human FOXD4L6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 208-417 aa of BC103887

Sequence: QLTPGAHLPHPFPLPAAHAALHNPHPGPLLGAPAPPQPVPGAYPNTAPGRRPYALLHPHPLRYLLLSAPVYAGAPKKAEGAALATPAPFPCCSPHLVLSLGRRARVWRRHREADASLSALRVLCKGSGERVQGLRRVCPRPRGATATCSSDHQACCIPRPLPLCCKCPPPPLLGQFCSNSSSIRRRTAPTAALPPRARCWAGTCRPRRRC

相同性解析による交差性が予測される生物種
Full Name forkhead box D4-like 6
Calculated molecular weight 417 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC103887
Gene Symbol FOXD4L6
Gene ID (NCBI) 653404
RRIDAB_2878988
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SYB3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Forkhead box protein D4-like 6(FOXD4L6)also named as FOXD4 like 6 is a 417 amino acid protein, which contains one fork head DNA-binding domain. FOXD4L6 localizes in the nucleus. The molecular weight of FOXD4L6 is 46kDa, but our experiment always detected a 60-70kDa protein by western blot.

Protocols

Product Specific Protocols
WB protocol for FOXD4L6 antibody 22081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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