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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using FOXD4 Polyclonal antibody (24835-1-AP)

    WB analysis of NIH/3T3 using 24835-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24835-1-AP (FOXD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using FOXD4 Polyclonal antibody (24835-1-AP)

    WB analysis of RAW 264.7 using 24835-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 24835-1-AP (FOXD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using FOXD4 Polyclonal antibody (24835-1-AP)

    WB analysis of U-937 using 24835-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 24835-1-AP (FOXD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FOXD4 Polyclonal antibody (24835-1-AP)

    WB analysis of Jurkat using 24835-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24835-1-AP (FOXD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FOXD4 Polyclonal antibody

FOXD4 Polyclonal Antibody for WB, ELISA
Cat No. 24835-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FKHL9, forkhead box D4, Forkhead box protein D4, Forkhead related protein FKHL9, FOXD4, FOXD4A, FREAC 5, FREAC5, Myeloid factor alpha

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, Jurkat cells, RAW 264.7 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24835-1-AP targets FOXD4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19226

Product name: Recombinant human FOXD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 361-439 aa of BC136570

Sequence: ILQQQQRHQEEDCANGCAPTKGAVLGGHLSAASALLRYQAVAEGSGLTSLAAPLGGEGTSPVFLVSPTPSSLAESAGPS

相同性解析による交差性が予測される生物種
Full Name forkhead box D4
Calculated molecular weight 439 aa, 47 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC136570
Gene Symbol FOXD4
Gene ID (NCBI) 2298
RRIDAB_2879750
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12950
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Forkhead box protein D4(FOXD4)also named as FKHL9 or Myeloid factor alpha is a 439 amino acid protein, which contains one fork head DNA-binding domain. FOXD4L6 localizes in the nucleus. FOXD4 as an embryonic transcriptional regulator involves in leukemogenesis. The molecular weight of FOXD4L6 is 47kDa, but our experiment always detected a 60-70kDa protein by western blot.

Protocols

Product Specific Protocols
WB protocol for FOXD4 antibody 24835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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