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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00470-4

    Sandwich ELISA standard curve of MP00470-4

    Sandwich ELISA standard curve of MP00470-4, Human FOXC2 Recombinant Matched Antibody Pair - PBS only. 83476-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33121. 83476-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP00470-4

    Sample test of MP00470-4

    The mean FOXC2 concentration was determined to be 2.8 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using FOXC2 Recombinant antibody (83476-5-RR)

    IF Staining of A375 using 83476-5-RR (same clone as 83476-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using FOXC2 antibody (83476-5-RR, Clone: 240403H7 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FOXC2 Recombinant antibody (83476-5-RR)

    FC experiment of HeLa using 83476-5-RR (same clone as 83476-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-5-RR, Clone:240403H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using FOXC2 Recombinant antibody (83476-5-RR)

    FC experiment of A549 using 83476-5-RR (same clone as 83476-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-5-RR, Clone:240403H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83476-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83476-5-RR

    Affinity and Kinetic Characterization of 83476-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83476-5-RR against Human FOXC2 were performed. The affinity constant is 2.79 nM.

FOXC2 Recombinant monoclonal antibody, PBS Only (Detector)

FOXC2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83476-5-PBS
Clone No.240403H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MFH 1, Mesenchyme fork head protein 1, Forkhead-related protein FKHL14, Forkhead box protein C2, FKHL14

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83476-5-PBS targets FOXC2 as part of a matched antibody pair:

MP00470-4: 83476-7-PBS capture and 83476-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag33121

Product name: Recombinant human FOXC2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 205-501 aa of NM_005251

Sequence: KEAEKKVVIKSEAASPALPVITKVETLSPESALQGSPRSAASTPAGSPDGSLPEHHAAAPNGLPGFSVENIMTLRTSPPGGELSPGAGRAGLVVPPLALPYAAAPPAAYGQPCAQGLEAGAAGGYQCSMRAMSLYTGAERPAHMCVPPALDEALSDHPSGPTSPLSALNLAAGQEGALAATGHHHQHHGHHHPQAPPPPPAPQPQPTPQPGAAAAQAASWYLNHSGDLNHLPGHTFAAQQQTFPNVREMFNSHRLGIENSTLGESQVSGNASCQLPYRSTPPLYRHAAPYSYDCTKY*

相同性解析による交差性が予測される生物種
Full Name forkhead box C2 (MFH-1, mesenchyme forkhead 1)
Calculated molecular weight54kd
GenBank accession numberNM_005251
Gene Symbol FOXC2
Gene ID (NCBI) 2303
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ99958
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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