Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HEK293 cells, L02 cells, HepG2 cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66568-1-Ig targets FOXC1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human | 
| Host / Isotype | Mouse / IgG1 | 
| Class | Monoclonal | 
| Type | Antibody | 
| Immunogen | 
                                             Peptide 相同性解析による交差性が予測される生物種 | 
                                    
| Full Name | forkhead box C1 | 
| Calculated molecular weight | 57 kDa | 
| Observed molecular weight | 70-75 kDa | 
| GenBank accession number | NM_001453 | 
| Gene Symbol | FOXC1 | 
| Gene ID (NCBI) | 2296 | 
| RRID | AB_2881929 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Protein G purification | 
| UNIPROT ID | Q12948 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Background Information
FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXC1 antibody 66568-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 

