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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    WB analysis using 12498-1-AP (same clone as 12498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IP experiment of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IP experiment of K-562 using 12498-1-AP (same clone as 12498-1-PBS)

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of human breast cancer using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of human placenta using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of human placenta using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of human lung cancer using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of human ovary tumor using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of human ovary tumor using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IHC staining of human lung cancer using 12498-1-AP (same clone as 12498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IF Staining of mouse brain using 12498-1-AP (same clone as 12498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FOX2/RBM9 Polyclonal antibody (12498-1-AP)

    IF Staining of HepG2 using 12498-1-AP (same clone as 12498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12498-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FOX2/RBM9 Polyclonal antibody, PBS Only

FOX2/RBM9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 12498-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

FOX2, RBM9, Fox 2, Fox-1 homolog B, fxh

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12498-1-PBS targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3168

Product name: Recombinant human RBM9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 6-358 aa of BC013115

Sequence: MVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQSSENSESKSTPKRLHVSNIPFRFRDPDLRQMFGGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKMVTPYANGWKLSPVVGAVYGPELYAASSFQADVSLGNDAAVPLSGRGGINTYIPLIIPGFPYPTAATTAAAFRGAHLRGRGRTVYGAVRAVPPTAIPAYPGVVYQDGFYGADLYGGYAAYRYAQPATATAATAAAAAVAAYSDGYGRVYTADPYHALAPAASYGVGAVASLYRGGYSRFAPY

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 9
Calculated molecular weight 390 aa, 41 kDa
Observed molecular weight 50- 60 kDa
GenBank accession numberBC013115
Gene Symbol RBM9
Gene ID (NCBI) 23543
RRIDAB_2877861
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43251
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)

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