Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13632-1-AP targets FNDC4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4550 Product name: Recombinant human FNDC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC032725 Sequence: MPSGCHSSPPSGLRGDMASLVPLSPYLSPTVLLLVSCDLGFVRADRPPSPVNVTVTHLRANSATVSWDVPEGNIVIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKGSDRLPSNSSSPGDITVEGLDGERPLQTGEVVIIVVVLLMWAAVIGLFCRQYDIIKDNDSNNNPKEKGKGPEQSPQGRPVGTRQKKSPSINTIDV 相同性解析による交差性が予測される生物種 |
| Full Name | fibronectin type III domain containing 4 |
| Calculated molecular weight | 234 aa, 25 kDa |
| Observed molecular weight | 21 kDa, 36 kDa |
| GenBank accession number | BC032725 |
| Gene Symbol | FNDC4 |
| Gene ID (NCBI) | 64838 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6D8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FNDC4 is a member of the fibronectin type III domain family of proteins. It is a secreted factor consisting of a C-terminal hydrophobic domain, a type III fibronectin domain, and an N-terminal membrane signaling region. FNDC4 is expressed at the highest levels in the liver and brain, but its expression levels are lower in many other tissues, including the heart, skeletal muscle, and adipose tissue. The N-terminal of FNDC4 can be cleaved, releasing the extracellular portion of the protein. (PMID: 32881638; 27066907)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FNDC4 antibody 13632-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |