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Validation Data Gallery

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  • Western Blot (WB) analysis of 4T1 cells using FNDC3B Polyclonal antibody (22605-1-AP)

    WB analysis of 4T1 using 22605-1-AP (same clone as 22605-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using FNDC3B Polyclonal antibody (22605-1-AP)

    WB analysis of C6 using 22605-1-AP (same clone as 22605-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using FNDC3B Polyclonal antibody (22605-1-AP)

    WB analysis of HL-60 using 22605-1-AP (same clone as 22605-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FNDC3B Polyclonal antibody (22605-1-AP)

    WB analysis of HepG2 using 22605-1-AP (same clone as 22605-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using FNDC3B Polyclonal antibody (22605-1-AP)

    IP experiment of HepG2 using 22605-1-AP (same clone as 22605-1-PBS)

    IP result of anti-FNDC3B (IP:22605-1-AP, 4ug; Detection:22605-1-AP 1:1000) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using FNDC3B Polyclonal antibody (22605-1-AP)

    IHC staining of human liver using 22605-1-AP (same clone as 22605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22605-1-AP (FNDC3B Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using FNDC3B Polyclonal antibody (22605-1-AP)

    IHC staining of human liver using 22605-1-AP (same clone as 22605-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22605-1-AP (FNDC3B Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22605-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FNDC3B Polyclonal antibody, PBS Only

FNDC3B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 22605-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22605-1-PBS targets FNDC3B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18357

Product name: Recombinant human FNDC3B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 3-370 aa of BC039297

Sequence: VTMMMTDQIPLELPPLLNGEVAMMPHLVNGDAAQQVILVQVNPGETFTIRAEDGTLQCIQGPAEVPMMSPNGSIPPIHVPPGYISQVIEDSTGVRRVVVTPQSPECYPPSYPSAMSPTHHLPPYLTHHPHFIHNSHTAYYPPVTGPGDMPPQFFPQHHLPHTIYGEQEIIPFYGMSSYITREDQYSKPPHKKLKDRQIDRQNRLNSPPSSIYKSSCTTVYNGYGKGHSGGSGGGGSGSGPGIKKTERRARSSPKSNDSDLQEYELEVKRVQDILSGIEKPQVSNIQARAVVLSWAPPVGLSCGPHSGLSFPYSYEVALSDKGRDGKYKIIYSGEELECNLKDLRPATDYHVRVYAMYNSVKGSCSEPV

相同性解析による交差性が予測される生物種
Full Name fibronectin type III domain containing 3B
Calculated molecular weight 1204 aa, 133 kDa
Observed molecular weight 150 kDa, 70 kDa
GenBank accession numberBC039297
Gene Symbol FNDC3B
Gene ID (NCBI) 64778
RRIDAB_2879133
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53EP0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fibronectin type III domain-containing protein 3B(FNDC3B), also named FAD104, belongs to the FNDC3 family. NDC3B is a positive regulator of adipocyte differentiation and is expressed at an early stage of adipocyte differentiation. FNDC3B is an endoplasmic reticulum transmembrane protein with a single transmembrane domain at the C terminus preceded by nine repeated fibronectin type III domains(PMID: 32966780). Due to the ability of the fibronectin type III domain to bind to a variety of proteins, FNDC3B plays a crucial role in cell adhesion, proliferation, and growth signaling. FNDC3B is abnormally expressed in a variety of human cancers, including hepatocellular carcinoma, acute myeloid leukemia, colorectal carcinoma, and cervical carcinoma(PMID: 36275754).

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