Validation Data Gallery

Filter: AllWBIPIHCFC (Intra)

WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66548-1-Ig (FMR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)
IP result of anti-FMR1 (IP:66548-1-Ig, 5ug; Detection:66548-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66548-1-Ig (same clone as 66548-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66548-1-Ig (FMR1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
×
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
FC experiment of Jurkat using 66548-1-Ig (same clone as 66548-1-PBS)
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.
×
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (66548-1-Ig, Clone:2B5B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66548-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

FMR1 Monoclonal antibody, PBS Only

FMR1 Monoclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Cat No. 66548-1-PBS

Clone No.2B5B5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

2B5B5, FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66548-1-PBS targets FMR1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag4697 Product name: Recombinant human FMR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC038998 Sequence: MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGLKI 相同性解析による交差性が予測される生物種
Full Name	fragile X mental retardation 1
Calculated molecular weight	632 aa, 71 kDa
Observed molecular weight	69-72 kDa
GenBank accession number	BC038998
Gene Symbol	FMR1
Gene ID (NCBI)	2332
RRID	AB_2881910
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q06787
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66548-1-Ig (same clone as 66548-1-PBS)

IP experiment of HeLa using 66548-1-Ig (same clone as 66548-1-PBS)

IHC staining of human gliomas using 66548-1-Ig (same clone as 66548-1-PBS)

IHC staining of human colon using 66548-1-Ig (same clone as 66548-1-PBS)