Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of Jurkat cells using 13755-1-AP
WB result of FMR1 antibody (13755-1-AP, 1:1500) with si-Control and si-FMR1 transfected Jurkat cells. FMR1 gene has many isoforms with MW 59-72 kDa.
WB analysis of K-562 using 13755-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13755-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13755-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13755-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13755-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13755-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 13755-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13755-1-AP
IP result of anti-FMR1 (IP:13755-1-AP, 4ug; Detection:13755-1-AP 1:1000) with HeLa cells lysate 2800ug.
IP experiment of HeLa cells using 13755-1-AP
IP result of anti-FMRP (13755-1-AP for IP and Detection).
IHC staining of human colon using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 13755-1-AP (FMRP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human gliomas using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 13755-1-AP (FMRP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13755-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13755-1-AP (FMRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13755-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FMR1 antibody (13755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HeLa using 13755-1-AP
Immunofluorescent analysis of Hela cells, using FMR1 antibody 13755-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HepG2 using 13755-1-AP
Immunofluorescent analysis of HepG2 cells using 13755-1-AP (FMRP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
FC experiment of Jurkat using 13755-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (13755-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 13755-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1, FRAXA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, mouse brain tissue, mouse thymus tissue, Jurkat cells, HeLa cells, HEK-293 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon tissue, human colon cancer tissue, human gliomas tissue, human liver tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

13755-1-AP targets FMR1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4697 Product name: Recombinant human FMR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC038998 Sequence: MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGLKI 相同性解析による交差性が予測される生物種
Full Name	fragile X mental retardation 1
Calculated molecular weight	632 aa, 71 kDa
Observed molecular weight	59-72 kDa
GenBank accession number	BC038998
Gene Symbol	FMR1
Gene ID (NCBI)	2332
RRID	AB_2262872
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q06787
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Protocols

Product Specific Protocols
IF protocol for FMR1 antibody 13755-1-AP	Download protocol
IHC protocol for FMR1 antibody 13755-1-AP	Download protocol
IP protocol for FMR1 antibody 13755-1-AP	Download protocol
WB protocol for FMR1 antibody 13755-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. Authors - David W Sanders View Article
human	WB	Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis Authors - Ji Geng View Article
human	WB	Cell Rep hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility Authors - Xiaoli Wang View Article
human	WB	Int Immunopharmacol MNSFβ promotes LPS-induced TNFα expression by increasing the localization of RC3H1 to stress granules, and the interfering peptide HEPN2 reduces TNFα production by disrupting the MNSFβ-RC3H1 interaction in macrophages Authors - Han-Yu Jiang View Article
human	WB	Cancer Cell Int MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome. Authors - Yu Men View Article
human	WB	Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. Authors - Olga Gourdomichali View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat cells using 13755-1-AP

WB analysis of K-562 using 13755-1-AP

WB analysis of HeLa using 13755-1-AP

WB analysis of HEK-293 using 13755-1-AP

WB analysis of mouse brain using 13755-1-AP

WB analysis of mouse thymus using 13755-1-AP

WB analysis of Jurkat using 13755-1-AP

WB analysis of K-562 using 13755-1-AP

IP experiment of HeLa using 13755-1-AP

IP experiment of HeLa cells using 13755-1-AP

IHC staining of human colon using 13755-1-AP

IHC staining of human colon using 13755-1-AP

IHC staining of human gliomas using 13755-1-AP

IHC staining of human gliomas using 13755-1-AP

IHC staining of human colon cancer using 13755-1-AP

IHC staining of human liver using 13755-1-AP

IHC staining of human colon cancer using 13755-1-AP

IHC staining of human liver using 13755-1-AP

IHC staining of mouse brain using 13755-1-AP

IF Staining of HepG2 using 13755-1-AP

IF Staining of HeLa using 13755-1-AP

IF Staining of HeLa using 13755-1-AP

IF Staining of HepG2 using 13755-1-AP

FC experiment of Jurkat using 13755-1-AP

FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

MNSFβ promotes LPS-induced TNFα expression by increasing the localization of RC3H1 to stress granules, and the interfering peptide HEPN2 reduces TNFα production by disrupting the MNSFβ-RC3H1 interaction in macrophages

MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome.

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.