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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMNL3 Polyclonal antibody (29756-1-AP)

    WB analysis using 29756-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29756-1-AP (FMNL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FMNL3 Polyclonal antibody (29756-1-AP)

    IHC staining of human colon cancer using 29756-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FMNL3 Polyclonal antibody (29756-1-AP)

    IHC staining of human colon cancer using 29756-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FMNL3 Polyclonal antibody (29756-1-AP)

    IHC staining of mouse liver using 29756-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FMNL3 Polyclonal antibody (29756-1-AP)

    IHC staining of mouse liver using 29756-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using FMNL3 Polyclonal antibody (29756-1-AP)

    IF Staining of HUVEC using 29756-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FMNL3 antibody (29756-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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FMNL3 Polyclonal antibody

FMNL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29756-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp762B245, FHOD3, FLJ45265, FMNL3, formin like 3, Formin like protein 3, KIAA2014, WBP 3, WBP3, WW domain binding protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29756-1-AP targets FMNL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMNL3 fusion protein Ag30543 相同性解析による交差性が予測される生物種
Full Name formin-like 3
Calculated molecular weight 117 kDa
Observed molecular weight117 kDa
GenBank accession numberNM_175736
Gene Symbol FMNL3
Gene ID (NCBI) 91010
RRIDAB_3086155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FMNL3 plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the control of cell shape and migration. In quiescent endothelial cells, FMNL3 triggers rearrangement of the actin cytoskeleton, but does not alter microtubule alignement.

Protocols

Product Specific Protocols
WB protocol for FMNL3 antibody 29756-1-APDownload protocol
IHC protocol for FMNL3 antibody 29756-1-APDownload protocol
IF protocol for FMNL3 antibody 29756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols