Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, L02 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13038-1-AP targets FMNL2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3692 Product name: Recombinant human FMNL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 880-1057 aa of BC036492 Sequence: LFYNELHYVEKAAAVSLENVLLDVKELQRGMDLTKREYTMHDHNTLLKEFILNNEGKLKKLQDDAKIAQDAFDDVVKYFGENPKTTPPSVFFPVFVRFVKAYKQAEEENELRKKQEQALMEKLLEQEALMEQQDPKSPSHKSKRQQQELIAELRRRQVKDNRHVYEGKDGAIEDIIT 相同性解析による交差性が予測される生物種 |
| Full Name | formin-like 2 |
| Calculated molecular weight | 1092 aa, 124 kDa |
| Observed molecular weight | 140-150 kDa |
| GenBank accession number | BC036492 |
| Gene Symbol | FMNL2 |
| Gene ID (NCBI) | 114793 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PY5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FMNL2 (Formin-like 2) is a member of the formin family of actin nucleators that promotes the assembly of unbranched actin filaments. It plays critical roles in cell migration, adhesion, and cytoskeletal remodeling by interacting with Rho GTPases and regulating actin dynamics at the leading edge of migrating cells (PMID: 38891874). Dysregulation of FMNL2 has been implicated in cancer metastasis and inflammatory disorders (PMID: 40212590). This antibody detects a band at 140-150 kDa, which is consistent with the results described in the literature (PMID: 24700756, PMID: 20633255).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FMNL2 antibody 13038-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |