Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse spleen tissue, PC-3 cells, HeLa cells, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
20712-1-AP targets VEGFR3/FLT4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, deer |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | fms-related tyrosine kinase 4 |
| Calculated molecular weight | 153 kDa |
| Observed molecular weight | 125 kDa |
| GenBank accession number | NM_002020 |
| Gene Symbol | FLT4 |
| Gene ID (NCBI) | 2324 |
| RRID | AB_10733474 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FLT4, also named VEGFR-3, belongs to the protein kinase superfamily, Tyr protein kinase family, and CSF-1/PDGF receptor subfamily. It is a receptor for VEGFC. FLT4 has a tyrosine-protein kinase activity. Defects in FLT4 cause lymphedema hereditary type 1 (LYH1A). Defects in FLT4 are found in juvenile hemangioma. VEGFR-3 is a highly glycosylated protein and migrates as bands with different molecular weights: an ~175 kDa precursor, an ~195 kDa mature form, an ~140 kDa non-glycosylated backbone, and a form that appears to be partially cleaved proteolytically in the extracellular domain to produce an ~125 kDa band(PMID: 15389531).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VEGFR3/FLT4 antibody 20712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma | ||
Int J Biol Sci PROTAC-Surufatinib Suppresses Pancreatic Neuroendocrine Neoplasms Progression by Inducing Ferroptosis through Inhibiting WNT/β-catenin Pathway Mediated by HMOX1 | ||
Front Oncol The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis. | ||
Stem Cell Res Ther Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration. | ||
Pharmaceuticals (Basel) Use of Early Donated COVID-19 Convalescent Plasma Is Optimal to Preserve the Integrity of Lymphatic Endothelial Cells. | ||
Mol Cell Endocrinol Long noncoding RNA GAS5 accelerates diabetic wound healing and promotes lymphangiogenesis via miR-217/Prox1 axis. |