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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FLT3 Polyclonal antibody (30297-1-AP)

    WB analysis using 30297-1-AP (same clone as 30297-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30297-1-AP (FLT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using FLT3 Polyclonal antibody (30297-1-AP)

    WB analysis of THP-1 using 30297-1-AP (same clone as 30297-1-PBS)

    WB result of FLT3 antibody (30297-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLT3 transfected THP-1 cells. This data was developed using the same antibody clone with 30297-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of THP-1 cells using FLT3 Polyclonal antibody (30297-1-AP)

    IP experiment of THP-1 using 30297-1-AP (same clone as 30297-1-PBS)

    IP result of anti-FLT3 (IP:30297-1-AP, 4ug; Detection:30297-1-AP 1:500) with THP-1 cells lysate 2720 ug. This data was developed using the same antibody clone with 30297-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using FLT3 Polyclonal antibody (30297-1-AP)

    IF Staining of HEK-293 using 30297-1-AP (same clone as 30297-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using FLT3 antibody (30297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30297-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FLT3 Polyclonal antibody, PBS Only

FLT3 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 30297-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

CD135, Fetal liver kinase-2, FL cytokine receptor, FLK2, FLK-2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30297-1-PBS targets FLT3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32898

Product name: Recombinant human FLT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 683-810 aa of BC126350

Sequence: LSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYENQKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHR

相同性解析による交差性が予測される生物種
Full Name fms-related tyrosine kinase 3
Calculated molecular weight112 kDa
Observed molecular weight130 kDa, 150 kDa
GenBank accession numberBC126350
Gene Symbol FLT3
Gene ID (NCBI) 2322
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP36888
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

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