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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FLJ23584 Polyclonal antibody (21143-1-AP)

    WB analysis of HepG2 using 21143-1-AP (same clone as 21143-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (FLJ23584 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    WB analysis of human brain using 21143-1-AP (same clone as 21143-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    WB analysis of mouse liver using 21143-1-AP (same clone as 21143-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using FLJ23584 Polyclonal antibody (21143-1-AP)

    WB analysis of L02 using 21143-1-AP (same clone as 21143-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    IHC staining of human brain using 21143-1-AP (same clone as 21143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    IHC staining of human brain using 21143-1-AP (same clone as 21143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    IHC staining of human testis using 21143-1-AP (same clone as 21143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 21143-1-AP (C22orf46 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    IHC staining of human testis using 21143-1-AP (same clone as 21143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 21143-1-AP (C22orf46 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FLJ23584 Polyclonal antibody (21143-1-AP)

    IF Staining of HepG2 using 21143-1-AP (same clone as 21143-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 21143-1-AP (FLJ23584 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21143-1-PBS in a different storage buffer formulation.

FLJ23584 Polyclonal antibody, PBS Only

FLJ23584 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21143-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21143-1-PBS targets FLJ23584 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15647

Product name: Recombinant human CTA-216E10.6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-234 aa of BC007210

Sequence: MNPVPHWGEVFLLVGGEGEHLASQGTTPARDHRVGISPASQQAQPESWRRRQRDKGVDPEKAPSLTRQSQNPPSLTAPLGMPSACSCLPCGPAPEAAIILAGPPTALTVLPKGTGLKKSKRLLLESLMRRRIAHLKWGLPRRILESYFLFNFLGSCSLTLAGARLSGLNTGQELQAQQERYCEAQGSPPGLKSPERFQRVQRPDRKSSKLPIQARALERNRPHMSEPIKHFHPA

相同性解析による交差性が予測される生物種
Full Name hypothetical FLJ23584
Calculated molecular weight 234 aa, 26 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC007210
Gene Symbol FLJ23584
Gene ID (NCBI) 79640
RRIDAB_10732689
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDC9J442
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FLJ23584 is coded by the mRNA of chromosome 22 open reading frame 46. The reading frame is different to C22orf46. This antibody detects the hypothetical FLJ23584 protein.

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