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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGEF40 Polyclonal antibody

ARHGEF40 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20110-1-AP

Synonyms

ARHGEF40, FLJ10357, hypothetical protein FLJ10357, Protein SOLO, SOLO

Validation Data Gallery

Applications:
  • WB analysis of SH-SY5Y using 20110-1-AP

    WB analysis of SH-SY5Y using 20110-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20110-1-AP (ARHGEF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 20110-1-AP

    WB analysis of mouse brain using 20110-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20110-1-AP (ARHGEF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Neuro-2a using 20110-1-AP

    WB analysis of Neuro-2a using 20110-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20110-1-AP (ARHGEF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 20110-1-AP

    WB analysis of HEK-293 using 20110-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20110-1-AP (ARHGEF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20110-1-AP

    IHC staining of human kidney using 20110-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20110-1-AP (ARHGEF40 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 20110-1-AP

    IHC staining of human kidney using 20110-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20110-1-AP (ARHGEF40 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 20110-1-AP

    IF Staining of SH-SY5Y using 20110-1-AP

    Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 20110-1-AP (ARHGEF40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, mouse brain tissue, Neuro-2a cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20110-1-AP targets ARHGEF40 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGEF40 fusion protein Ag13886 相同性解析による交差性が予測される生物種
Full Name hypothetical protein FLJ10357
Calculated molecular weight 414 aa, 46 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC000084
Gene symbol FLJ10357
Gene ID (NCBI) 55701
RRIDAB_10695049
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGEF40 antibody 20110-1-APDownload protocol
IHC protocol for ARHGEF40 antibody 20110-1-APDownload protocol
IF protocol for ARHGEF40 antibody 20110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols