Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, A549 cells, NCI-H1299 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells, HepG2 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
67039-1-Ig targets Flightless I in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Flightless I fusion protein Ag26865 相同性解析による交差性が予測される生物種 |
| Full Name | flightless I homolog (Drosophila) |
| Calculated molecular weight | 1269 aa, 145 kDa |
| Observed molecular weight | 145-150 kDa |
| GenBank accession number | BC025300 |
| Gene Symbol | Flightless I |
| Gene ID (NCBI) | 2314 |
| RRID | AB_2882353 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13045 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Flightless I antibody 67039-1-Ig | Download protocol |
| IHC protocol for Flightless I antibody 67039-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein | ||
Am J Cancer Res SH3BGRL2 exerts a dual function in breast cancer growth and metastasis and is regulated by TGF-β1. |