Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of HeLa using 11173-1-AP
WB result of FKBP8 antibody (11173-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells.
WB analysis using 11173-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11173-1-AP
WB result of FKBP8 antibody (11173-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells.
WB analysis of mouse brain using 11173-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11173-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 11173-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11173-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 11173-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11173-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11173-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11173-1-AP
IP result of anti-FKBP8 (IP:11173-1-AP, 4ug; Detection:11173-1-AP 1:500) with HeLa cells lysate 3200ug.
IF Staining of A431 using 11173-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using FKBP8 antibody (11173-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

FKBP8 Polyclonal antibody

FKBP8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 11173-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

FK506 binding protein 8, FK506-binding protein 8, FKBP 38, FKBP 8, FKBP38

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse pancreas tissue, Jurkat cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

11173-1-AP targets FKBP8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1649 Product name: Recombinant human FKBP8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC009966 Sequence: MGQPPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNGLLRKKTLVPGPPGSSRPVKGQVVTVHLQTSLENGTRVQEEPELVFTLGDCDVIQALDLSVPLMDVGETAMVTADSKYCYGPQGRSPYIPPHAALCLEVTLKTAVDGPDLEMLTGQERVALANRKRECGNAHYQRADFVLAANSYDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALRSCSLVLEHQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIHAELSKLVKKHAAQRSTETALYRKMLGNPSRLPAKCPGKGAWSIPWKWLFGATAVALGGVALSVVIAARN 相同性解析による交差性が予測される生物種
Full Name	FK506 binding protein 8, 38kDa
Calculated molecular weight	45 kDa
Observed molecular weight	53 kDa
GenBank accession number	BC009966
Gene Symbol	FKBP8
Gene ID (NCBI)	23770
RRID	AB_10597097
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14318
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for FKBP8 antibody 11173-1-AP	Download protocol
IP protocol for FKBP8 antibody 11173-1-AP	Download protocol
WB protocol for FKBP8 antibody 11173-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IP	Elife ZMYND10 functions in a chaperone relay during axonemal dynein assembly. Authors - Girish R Mali View Article
human		Front Bioeng Biotechnol Efficient and reversible Cas13d-mediated knockdown with an all-in-one lentivirus-vector Authors - Suli Lv KO Validated View Article
rat	IF	Int J Mol Sci Analysis of Dysferlin Direct Interactions with Putative Repair Proteins Links Apoptotic Signaling to Ca2+ Elevation via PDCD6 and FKBP8 Authors - Dennis G Drescher View Article
human	WB	J Biol Chem Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma Authors - Suli Lv KO Validated View Article
human	WB	Mol Hum Reprod Comprehensive analysis of ovarian granulosa cell proteomics and phosphoproteomics in PCOS patients without insulin resistance Authors - Xiao Yang View Article
human	WB	Cell Chem Biol Integrative proximal-ubiquitomics profiling for deubiquitinase substrate discovery applied to USP30 Authors - Andreas Damianou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 11173-1-AP

WB analysis using 11173-1-AP

WB analysis of HeLa using 11173-1-AP

WB analysis of mouse brain using 11173-1-AP

WB analysis of mouse liver using 11173-1-AP

WB analysis of SH-SY5Y using 11173-1-AP

WB analysis of mouse brain using 11173-1-AP

WB analysis of mouse pancreas using 11173-1-AP

WB analysis of Jurkat using 11173-1-AP

WB analysis of HeLa using 11173-1-AP

IP experiment of HeLa using 11173-1-AP

IF Staining of A431 using 11173-1-AP

FKBP8 Polyclonal antibody

FKBP8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ZMYND10 functions in a chaperone relay during axonemal dynein assembly.

Efficient and reversible Cas13d-mediated knockdown with an all-in-one lentivirus-vector

Analysis of Dysferlin Direct Interactions with Putative Repair Proteins Links Apoptotic Signaling to Ca2+ Elevation via PDCD6 and FKBP8

Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma

Comprehensive analysis of ovarian granulosa cell proteomics and phosphoproteomics in PCOS patients without insulin resistance

Integrative proximal-ubiquitomics profiling for deubiquitinase substrate discovery applied to USP30