• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of PC-3 cells using FKBP52 Polyclonal antibody (22623-1-AP)

    WB analysis of PC-3 using 22623-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22623-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using FKBP52 Polyclonal antibody (22623-1-AP)

    IHC staining of human colon using 22623-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22623-1-AP (FKBP52 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using FKBP52 Polyclonal antibody (22623-1-AP)

    IHC staining of human colon using 22623-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22623-1-AP (FKBP52 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FKBP52 Polyclonal antibody (22623-1-AP)

    IHC staining of human kidney using 22623-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FKBP52 Polyclonal antibody (22623-1-AP)

    IHC staining of human kidney using 22623-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FKBP52 Polyclonal antibody (22623-1-AP)

    IF Staining of PC-3 using 22623-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using FKBP52 antibody (22623-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FKBP52 Polyclonal antibody (22623-1-AP)

    IF Staining of PC-3 using 22623-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 22623-1-AP (FKBP52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FKBP52 Polyclonal antibody

FKBP52 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22623-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FKBP4, FKBP-4, FK506-binding protein 4, EC:5.2.1.8, 52 kDa FK506-binding protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22623-1-AP targets FKBP52 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18704

Product name: Recombinant human FKBP52 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-286 aa of BC007924

Sequence: MTAEEMKATESGAQSAPLPMEGVDISPKQDEGVLKVIKREGTGTEMPMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFDLGKGEVIKAWDIAIATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEEEDGGIIRRIQTRGEGYAKPNEGAIVEVALEGYYKDKLFDQRELRFEIGEGENLDLPYGLERAIQRMEKGEHSIVYLKPSYAFGSVGKEKFQIPPNAELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKY

相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 4, 59kDa
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007924
Gene Symbol FKBP52
Gene ID (NCBI) 2288
RRIDAB_2879136
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ02790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The large molecular-weight immunophilin, FKBP52, is a known target of the immunosuppressive drug FK506. FKBP52 exhibits peptidyl-prolyl cis-trans isomerase (PPIase) activity, which is inhibited by the binding of FK506-properties that it shares with the smaller but better-studied immunophilin, FKBP12(PMID:15381148). It is essential to AR-mediated induction of genes involved in sexually dimorphic development of the penis (NKX3-1), in erectile function of the mature organ (NOS2 and NOS3), and in promotion of apoptosis during development(PMID:20605780).

Protocols

Product Specific Protocols
IF protocol for FKBP52 antibody 22623-1-APDownload protocol
IHC protocol for FKBP52 antibody 22623-1-APDownload protocol
WB protocol for FKBP52 antibody 22623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...