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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP1A Recombinant antibody (86249-1-RR)

    WB analysis using 86249-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86249-1-RR (FKBP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FKBP1A Recombinant antibody (86249-1-RR)

    WB analysis using 86249-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86249-1-RR (FKBP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86249-1-RR

    Affinity and Kinetic Characterization of 86249-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86249-1-RR against Human FKBP1A were performed. The affinity constant is 0.899 nM.

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FKBP1A Recombinant antibody

FKBP1A Recombinant Antibody for WB, ELISA
Cat No. 86249-1-RR
Clone No.250923G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

12 kDa FK506-binding protein, Calstabin-1, FK506-binding protein 1A, FKBP 1A, FKBP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, U-87 MG cells, PANC-1 cells, Neuro-2a cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86249-1-RR targets FKBP1A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FKBP1A fusion protein Ag0406 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 1A, 12kDa
Calculated molecular weight 12 kDa
Observed molecular weight15 kDa
GenBank accession numberBC001925
Gene Symbol FKBP1A
Gene ID (NCBI) 2280
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP1A(FK506-binding protein 1A) is also named as FKBP1, FKBP12 and belongs to the FKBP-type PPIase family.It keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Protocols

Product Specific Protocols
WB protocol for FKBP1A antibody 86249-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols