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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using FJX1 Polyclonal antibody (17417-1-AP)

    WB analysis of rat brain using 17417-1-AP (same clone as 17417-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17417-1-AP (FJX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of human liver using 17417-1-AP (same clone as 17417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of mouse brain using 17417-1-AP (same clone as 17417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of human liver using 17417-1-AP (same clone as 17417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of mouse brain using 17417-1-AP (same clone as 17417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of mouse testis using 17417-1-AP (same clone as 17417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of mouse testis using 17417-1-AP (same clone as 17417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17417-1-PBS in a different storage buffer formulation.

FJX1 Polyclonal antibody, PBS Only

FJX1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17417-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17417-1-PBS targets FJX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11385

Product name: Recombinant human FJX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 43-437 aa of BC049171

Sequence: EDRLPRRPARSGGPAPAPRFPLPPPLAWDARGGSLKTFRALLTLAAGADGPPRQSRSEPRWHVSARQPRPEESAAVHGGVFWSRGLEEQVPPGFSEAQAAAWLEAARGARMVALERGGCGRSSNRLARFADGTRACVRYGINPEQIQGEALSYYLARLLGLQRHVPPLALARVEARGAQWAQVQEELRAAHWTEGSVVSLTRWLPNLTDVVVPAPWRSEDGRLRPLRDAGGELANLSQAELVDLVQWTDLILFDYLTANFDRLVSNLFSLQWDPRVMQRATSNLHRGPGGALVFLDNEAGLVHGYRVAGMWDKYNEPLLQSVCVFRERTARRVLELHRGQDAAARLLRLYRRHEPRFPELAALADPHAQLLQRRLDFLAKHILHCKAKYGRRSGT

相同性解析による交差性が予測される生物種
Full Name four jointed box 1 (Drosophila)
Calculated molecular weight 437 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC049171
Gene Symbol FJX1
Gene ID (NCBI) 24147
RRIDAB_2878406
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VR8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Four-jointed box protein 1 (FJX1), also named as FLJ22416, is a 437 amino acid protein, which belongs to the FJX1 family. FJX1 is a secreted protein and acts as an inhibitor of dendrite and branching. FJX1 is important for growth and differentiation of legs and wings, and for proper development of the eyes. FAT4, an essential gene that has a key role in vertebrate PCP (planar cell polarity), represses FJX1 expression which may lead to cystic diseases in humans.

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