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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Monoclonal antibody

FITC Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

FITC

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C10A1

Cat no : 68132-1-Ig

Synonyms

Validation Data Gallery

Applications:
  • WB analysis using 68132-1-Ig

    WB analysis using 68132-1-Ig

    WB result of FITC antibody (68132-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with FITC conjugated protein and un-conjugated protein as negative control. Antibody to TDP-43 as the positive control to target protein.

  • WB analysis of BSA using 68132-1-Ig

    WB analysis of BSA using 68132-1-Ig

    WB results of FITC antibody (68132-1-Ig, 1:10000) with BSA and FITC conjugated BSA.

  • ELISA experiment of FITC conjugated antibody using 68132-1-Ig

    ELISA experiment of FITC conjugated antibody using 68132-1-Ig

    Indirect ELISA and competitive ELISA results show that this antibody is specific to FITC. Indirect ELISA was performed by coating FITC conjugated BSA at 0ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection (red curve, refer to top X-right Y axis). Competitive ELISA was performed similarly except that different concentration of FITC (active group been quenched) was mixed in 3.3ng/mL primary antibody (blue curve, refere to bottom X-left Y axis).

Tested Applications

Positive WB detected inFITC-TDP43, FITC-Ag1231, BSA
Positive ELISA detected inFITC conjugated antibody

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
Sample-dependent, check data in validation data gallery

Product Information

68132-1-Ig targets FITC in WB, ELISA applications and shows reactivity with FITC samples.

Tested Reactivity FITC
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Compound 相同性解析による交差性が予測される生物種
Full Name FITC
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Protocols

Product Specific Protocols
WB protocol for FITC antibody 68132-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols