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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FIS1 Recombinant antibody (82248-1-RR)

    WB analysis using 82248-1-RR (same clone as 82248-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82248-1-RR (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using FIS1 Recombinant antibody (82248-1-RR)

    IP experiment of HeLa using 82248-1-RR (same clone as 82248-1-PBS)

    IP result of anti-FIS1 (IP:82248-1-RR, 4ug; Detection:82248-1-RR 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FIS1 Recombinant antibody (82248-1-RR)

    IHC staining of human prostate cancer using 82248-1-RR (same clone as 82248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FIS1 Recombinant antibody (82248-1-RR)

    IHC staining of rat brain using 82248-1-RR (same clone as 82248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FIS1 Recombinant antibody (82248-1-RR)

    IF Staining of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FIS1 antibody (82248-1-RR, Clone: 6N15 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using FIS1 Recombinant antibody (82248-1-RR)

    FC experiment of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FIS1 (82248-1-RR, Clone:6N15) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82248-1-RR

    Affinity and Kinetic Characterization of 82248-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82248-1-RR against Human FIS1 were performed. The affinity constant is 0.251 nM.

FIS1 Recombinant monoclonal antibody, PBS Only

FIS1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 82248-1-PBS
Clone No.6N15

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135, CGI 135

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82248-1-PBS targets FIS1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1409

Product name: Recombinant human FIS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC009428

Sequence: MEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDGLVGMAIVGGMALGVAGLAGLIGLAVSKSKS

相同性解析による交差性が予測される生物種
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight15 kDa
GenBank accession numberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (PMID: 21257639, 21459773, 23333625)

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