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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FIS1 Recombinant antibody, PBS Only

FIS1 Recombinant Antibody for WB, IP, IF, FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6N15

Cat no : 82248-1-PBS

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

Validation Data Gallery

Applications:
  • WB analysis using 82248-1-RR (same clone as 82248-1-PBS)

    WB analysis using 82248-1-RR (same clone as 82248-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82248-1-RR (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82248-1-RR (same clone as 82248-1-PBS)

    IP experiment of HeLa using 82248-1-RR (same clone as 82248-1-PBS)

    IP result of anti-FIS1 (IP:82248-1-RR, 4ug; Detection:82248-1-RR 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82248-1-RR (same clone as 82248-1-PBS)

    IHC staining of human prostate cancer using 82248-1-RR (same clone as 82248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 82248-1-RR (same clone as 82248-1-PBS)

    IHC staining of rat brain using 82248-1-RR (same clone as 82248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82248-1-RR (FIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    IF Staining of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FIS1 antibody (82248-1-RR, Clone: 6N15 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    FC experiment of HepG2 using 82248-1-RR (same clone as 82248-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FIS1 (82248-1-RR, Clone:6N15) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82248-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

82248-1-PBS targets FIS1 in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FIS1 fusion protein Ag1409 相同性解析による交差性が予測される生物種
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight15 kDa
GenBank accession numberBC009428
Gene symbol FIS1
Gene ID (NCBI) 51024
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (PMID: 21257639, 21459773, 23333625)