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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FILIP1L Polyclonal antibody

FILIP1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30134-1-AP

Synonyms

COL4A3BPIP, DOC 1, DOC1, FILIP1L, GIP90

Validation Data Gallery

Applications:
  • WB analysis using 30134-1-AP

    WB analysis using 30134-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30134-1-AP (FILIP1L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 30134-1-AP

    IHC staining of mouse ovary using 30134-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30134-1-AP (FILIP1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse ovary using 30134-1-AP

    IHC staining of mouse ovary using 30134-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 30134-1-AP (FILIP1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse colon tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30134-1-AP targets FILIP1L in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FILIP1L fusion protein Ag32655 相同性解析による交差性が予測される生物種
Full Name filamin A interacting protein 1-like
Calculated molecular weight 893 aa, 102 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC027860
Gene symbol FILIP1L
Gene ID (NCBI) 11259
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FILIP1L antibody 30134-1-APDownload protocol
IHC protocol for FILIP1L antibody 30134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols