Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28499-1-AP targets FIG4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28971 Product name: Recombinant human FIG4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 654-792 aa of BC041338 Sequence: VNLKKLIVKKFHKYEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARDFMPKTVGIDPSPFTVRKPDETGKSVLGNKSNREEAVLQRKTAASAPPPPSEEAVSSSSEDDSGTDREEEGSVSQRSTPVKMTDAGDSAKVTE 相同性解析による交差性が予測される生物種 |
| Full Name | FIG4 homolog (S. cerevisiae) |
| Calculated molecular weight | 907 aa, 104 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC041338 |
| Gene Symbol | FIG4 |
| Gene ID (NCBI) | 9896 |
| RRID | AB_3742294 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92562 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FIG4 (also named SAC3) is a 5'-phosphoinositide phosphatase that coordinates the turnover of phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2), a very low abundance phosphoinositide. Deficiency of FIG4 severely affects the human and mouse nervous systems by causing two distinct forms of abnormal lysosomal storage (PMID: 23165282).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FIG4 antibody 28499-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |