Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31744-1-AP targets FHOD3 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36247 Product name: Recombinant human FHOD3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 650-825 aa of BC157093 Sequence: RTTGLWPAGVQDAGVNGQCGDILTNKRFMLDMLYAHNRKSPDDEEKGDGEAGRTQQEAEAVASLATRISTLQANSQTQDESVRRVDVGCLDNRGSVKAFAEKFNSGDLGRGSISPDAEPNDKVPETAPVQPKTESDYIWDQLMANPRELRIQDMDFTDLGEEDDIDVLDVDLGHRE 相同性解析による交差性が予測される生物種 |
| Full Name | formin homology 2 domain containing 3 |
| GenBank accession number | BC157093 |
| Gene Symbol | FHOD3 |
| Gene ID (NCBI) | 80206 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q2V2M9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FHOD3, also known as FHOS2 or Formactin2, was an important member of Formin homology proteins. FHOD3 plays a crucial role in organizing the cytoskeleton, primarily by regulating actin filament polymerization within cardiomyocytes, which is essential for heart muscle structure and function. Mutations in this gene are associated with familial cardiomyopathies, including dilated cardiomyopathy (DCM), and its protein levels are altered in hypertrophic cardiomyopathy (HCM). FHOD3 is expressed abundantly in the heart (PMID: 32447646, PMID: 33251537).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FHOD3 antibody 31744-1-AP | Download protocol |
| IHC protocol for FHOD3 antibody 31744-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |