Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 10991-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10991-1-AP
WB result of FHL1 antibody (10991-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and sg-FHL1 transfected HEK-293 cells.
WB analysis of human brain using 10991-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 10991-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10991-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10991-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10991-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10991-1-AP (FHL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 10991-1-AP
IP result of anti-FHL1 (IP:10991-1-AP, 4ug; Detection:10991-1-AP 1:8000) with mouse skeletal muscle tissue lysate 4000 ug.
IHC staining of human ovary tumor using 10991-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary tumor using 10991-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10991-1-AP (FHL1 antibody) at dilution of 1:50 (under 10x lens).

Featured Product
KD/KO Validated

FHL1 Polyclonal antibody

FHL1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10991-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

FHL 1, FHL-1, FHL1B, FLH1A, four and a half LIM domains 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (32)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, HEK-293 cells, human brain tissue, mouse colon tissue, rat skeletal muscle tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 27 publications below
IHC	See 10 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 2 publications below
ChIP	See 1 publications below

Product Information

10991-1-AP targets FHL1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity	human, mouse, rat, zebrafish
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1448 Product name: Recombinant human FHL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC010998 Sequence: MAEKFDCHYCRDPLQGKKYVQKDGHHCCLKCFDKFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETFVAKDNKILCNKCTTREDSPKCKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQVIGTGSFFPKGEDFYCVTCHETKFAKHCVKCNKAITSGGITYQDQPWHADCFVCVTCSKKLAGQRFTAVEDQYYCVDCYKNFVAKKCAGCKNPITGFGKGSSVVAYEGQSWHDYCFHCKKCSVNLANKRFVFHQEQVYCPDCAKKL 相同性解析による交差性が予測される生物種
Full Name	four and a half LIM domains 1
Calculated molecular weight	32 kDa
Observed molecular weight	32 kDa
GenBank accession number	BC010998
Gene Symbol	FHL1
Gene ID (NCBI)	2273
RRID	AB_2104230
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13642
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Four-and-a-half LIM domains 1 (FHL1), a member of the FHL protein family, which composed of FHL 1, 2, 3, 4 and ACT (activator of CREM), and characterized by an N-terminal half LIM domain followed by four complete LIM domains. FHL1 may contribute to sarcomere synthesis, assembly and biomechanical stress sensing. FHL1 is also a methylation-silenced tumor-suppressor gene on chromosome X in gastrointestinal cancers, which contributes to the formation of an epigenetic field for cancerization. FHL1(10991-1-AP) is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. This is a rabbit polyantibody raised against full length of human FHL1.

Protocols

Product Specific Protocols
IHC protocol for FHL1 antibody 10991-1-AP	Download protocol
IP protocol for FHL1 antibody 10991-1-AP	Download protocol
WB protocol for FHL1 antibody 10991-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,CoIP,ChIP	J Clin Invest Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway. Authors - Ding Lihua L KD Validated View Article
human	WB	Genes Dev Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers. Authors - Anne Loft KD Validated View Article
human	WB,IHC	Nat Commun A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Authors - Xiaojie Xu KD Validated View Article
		J Cell Biol Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter. Authors - Xiang Wang View Article
human	WB,IHC	Int J Cancer Downregulation and growth inhibitory role of FHL1 in lung cancer. Authors - Niu Chang C View Article
mouse,human	WB	J Cell Sci MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins. Authors - Leitner Laura L View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10991-1-AP

WB analysis of HEK-293 using 10991-1-AP

WB analysis of human brain using 10991-1-AP

WB analysis of mouse colon using 10991-1-AP

WB analysis of mouse skeletal muscle using 10991-1-AP

WB analysis of mouse skeletal muscle using 10991-1-AP

WB analysis of mouse skeletal muscle using 10991-1-AP

IP experiment of mouse skeletal muscle using 10991-1-AP

IHC staining of human ovary tumor using 10991-1-AP

IHC staining of human ovary tumor using 10991-1-AP

FHL1 Polyclonal antibody

FHL1 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.

Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter.

Downregulation and growth inhibitory role of FHL1 in lung cancer.

MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.