Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
83710-1-PBS targets FHIT in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4181 Product name: Recombinant human FHIT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC032336 Sequence: MSFRFGQHLIKPSVVFLKTELSFALVNRKPVVPGHVLVCPLRPVERFHDLRPDEVADLFQTTQRVGTVVEKHFHGTSLTFSMQDGPEAGQTVKHVHVHVLPRKAGDFHRNDSIYEELQKHDKEDFPASWRSEEEMAAEAAALRVYFQ 相同性解析による交差性が予測される生物種 |
| Full Name | fragile histidine triad gene |
| Calculated molecular weight | 147 aa, 17 kDa |
| Observed molecular weight | 15-17 kDa |
| GenBank accession number | BC032336 |
| Gene Symbol | FHIT |
| Gene ID (NCBI) | 2272 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P49789 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.
