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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FH Recombinant monoclonal antibody (82508-1-RR)

    WB analysis using 82508-1-RR (same clone as 82508-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82508-1-RR (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FH Recombinant monoclonal antibody (82508-1-RR)

    WB analysis of HepG2 using 82508-1-RR (same clone as 82508-1-PBS)

    WB result of FH antibody (82508-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using FH Recombinant monoclonal antibody (82508-1-RR)

    IP experiment of mouse liver using 82508-1-RR (same clone as 82508-1-PBS)

    IP result of anti-FH (IP:82508-1-RR, 4ug; Detection:82508-1-RR 1:4000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FH Recombinant monoclonal antibody (82508-1-RR)

    IHC staining of human stomach using 82508-1-RR (same clone as 82508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82508-1-RR (FH antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FH Recombinant monoclonal antibody (82508-1-RR)

    IHC staining of human stomach cancer using 82508-1-RR (same clone as 82508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82508-1-RR (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82508-1-RR

    Affinity and Kinetic Characterization of 82508-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82508-1-RR against Human FH were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

FH Recombinant monoclonal antibody, PBS Only

FH Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82508-1-PBS
Clone No.3K16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

3K16, EC:4.2.1.2, Fumarase, HsFH

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82508-1-PBS targets FH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1934

Product name: Recombinant human FH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 204-510 aa of BC003108

Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

相同性解析による交差性が予測される生物種
Full Name fumarate hydratase
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight45 kDa
GenBank accession numberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
RRIDAB_3086485
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP07954
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

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