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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FH Recombinant antibody (82508-1-RR)

    WB analysis using 82508-1-RR (same clone as 82508-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82508-1-RR (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FH Recombinant antibody (82508-1-RR)

    WB analysis of HepG2 using 82508-1-RR (same clone as 82508-1-PBS)

    WB result of FH antibody (82508-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using FH Recombinant antibody (82508-1-RR)

    IP experiment of mouse liver using 82508-1-RR (same clone as 82508-1-PBS)

    IP result of anti-FH (IP:82508-1-RR, 4ug; Detection:82508-1-RR 1:4000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FH Recombinant antibody (82508-1-RR)

    IHC staining of human stomach using 82508-1-RR (same clone as 82508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82508-1-RR (FH antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FH Recombinant antibody (82508-1-RR)

    IHC staining of human stomach cancer using 82508-1-RR (same clone as 82508-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82508-1-RR (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82508-1-RR

    Affinity and Kinetic Characterization of 82508-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82508-1-RR against Human FH were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

FH Recombinant monoclonal antibody, PBS Only

FH Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82508-1-PBS
Clone No.3K16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

3K16, EC:4.2.1.2, Fumarase, HsFH

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82508-1-PBS targets FH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1934

Product name: Recombinant human FH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 204-510 aa of BC003108

Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

相同性解析による交差性が予測される生物種
Full Name fumarate hydratase
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight45 kDa
GenBank accession numberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP07954
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

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