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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGFR3 Polyclonal antibody (30202-1-AP)

    WB analysis using 30202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30202-1-AP (FGFR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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FGFR3 Polyclonal antibody

FGFR3 Polyclonal Antibody for WB, ELISA
Cat No. 30202-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

ACH, CD333, CEK2, FGFR 3, HSFGFR3EX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30202-1-AP targets FGFR3 in applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor 3
Calculated molecular weight88kd
Observed molecular weight115 kDa
Gene Symbol FGFR3
Gene ID (NCBI) 2261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22607
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3, and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, the FGFR3 gene has also been associated with tumor progression.

Protocols

Product Specific Protocols
WB protocol for FGFR3 antibody 30202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols