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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FGFR2/CD332 Polyclonal antibody (13042-1-AP)

    IHC staining of human breast cancer using 13042-1-AP (same clone as 13042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FGFR2/CD332 Polyclonal antibody (13042-1-AP)

    IHC staining of human breast cancer using 13042-1-AP (same clone as 13042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FGFR2/CD332 Polyclonal antibody (13042-1-AP)

    IHC staining of human prostate cancer using 13042-1-AP (same clone as 13042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FGFR2/CD332 Polyclonal antibody (13042-1-AP)

    IHC staining of human prostate cancer using 13042-1-AP (same clone as 13042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FGFR2/CD332 Polyclonal antibody (13042-1-AP)

    IF Staining of HeLa using 13042-1-AP (same clone as 13042-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FGFR2 antibody (13042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FGFR2/CD332 Polyclonal antibody (13042-1-AP)

    IF Staining of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 13042-1-AP (FGFR2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FGFR2/CD332 Polyclonal antibody (13042-1-AP)

    IF Staining of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using FGFR2 antibody 13042-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using FGFR2/CD332 Polyclonal antibody (13042-1-AP)

    FC experiment of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human FGFR2 (13042-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13042-1-PBS targets FGFR2/CD332 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGFR2/CD332 fusion protein Ag3701 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor 2
Calculated molecular weight 709 aa, 79 kDa
Observed molecular weight
GenBank accession numberBC039243
Gene Symbol FGFR2
Gene ID (NCBI) 2263
RRIDAB_10642943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21802
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. This polyclonal antibody raised against 358-704aa of human FGFR2 can cross-react with other members of the FGFR family.