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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGFR1OP Polyclonal antibody (11343-1-AP)

    WB analysis using 11343-1-AP (same clone as 11343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FGFR1OP Polyclonal antibody (11343-1-AP)

    WB analysis of HEK-293 using 11343-1-AP (same clone as 11343-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using FGFR1OP Polyclonal antibody (11343-1-AP)

    WB analysis of mouse heart using 11343-1-AP (same clone as 11343-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11343-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using FGFR1OP Polyclonal antibody (11343-1-AP)

    WB analysis of U-937 using 11343-1-AP (same clone as 11343-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11343-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using FGFR1OP Polyclonal antibody (11343-1-AP)

    IHC staining of human lymphoma using 11343-1-AP (same clone as 11343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11343-1-AP (FGFR1OP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FGFR1OP Polyclonal antibody (11343-1-AP)

    IF Staining of HeLa using 11343-1-AP (same clone as 11343-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FGFR1OP antibody (11343-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11343-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using FGFR1OP Polyclonal antibody (11343-1-AP)

    IF Staining of HEK-293 using 11343-1-AP (same clone as 11343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using FGFR1OP antibody (11343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11343-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FGFR1OP Polyclonal antibody, PBS Only

FGFR1OP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11343-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FOP, FGFR1 oncogene partner, CEP43, Centrosomal protein 43

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11343-1-PBS targets FGFR1OP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1891

Product name: Recombinant human FGFR1OP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-374 aa of BC011902

Sequence: MAATAAAVVAEEDTELRDLLVQTLENSGVLNRIKAELRAAVFLALEEQEKVENKTPLVNESLRKFLNTKDGRLVASLVAEFLQFFNLDFTLAVFQPETSTLQGLEGRENLARDLGIIEAEGTVGGPLLLEVIRRCQQKEKGPTTGEGALDLSDVHSPPKSPEGKTSAQTTPSKKANDEANQSDTSVSLSEPKSKSSLHLLSHETKIGSFLSNRTLDGKDKAGLCPDEDDMEGDSFFDDPIPKPEKTYGLRNEPRKQAGSLASLSDAPPLKSGLSSLAGAPSLKDSESKRGNTVLKDLKLISDKIGSLGLGTGEDDDYVDDFNSTSHRSEKSEISIGEEIEEDLSVEIDDINTSDKLDDLTQDLTVSQLSDVADY

相同性解析による交差性が予測される生物種
Full Name FGFR1 oncogene partner
Calculated molecular weight 43 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC011902
Gene Symbol FGFR1OP
Gene ID (NCBI) 11116
RRIDAB_2103362
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95684
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The fibroblast growth factor receptor 1 oncogene partner (FGFR1OP), FOP, is a centrosomal protein that is involved in the anchoring of microtubules to subcellular structures. Three isoforms have been produced by alternative splicing. A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma and progresses to acute myeloid leukemia.