Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24725-1-AP targets FGFBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20573 Product name: Recombinant human FGFBP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-258 aa of BC025966 Sequence: MTPPKLRASLSPSLLLLLSGCLLAAARREKGAASNVAEPVPGPTGGSSGRFLSPEQHACSWQLLLPAPEAAAGSELALRCQSPDGARHQCAYRGHPERCAAYAARRAHFWKQVLGGLRKKRRPCHDPAPLQARLCAGKKGHGAELRLVPRASPPARPTVAGFAGESKPRARNRGRTRERASGPAAGTPPPQSAPPKENPSERKTNEGKRKAALVPNEERPMGTGPDPDGLDGNAELTETYCAEKWHSLCNFFVNFWNG 相同性解析による交差性が予測される生物種 |
| Full Name | fibroblast growth factor binding protein 3 |
| Calculated molecular weight | 258 aa, 28 kDa |
| Observed molecular weight | 60-65 kda |
| GenBank accession number | BC025966 |
| Gene Symbol | FGFBP3 |
| Gene ID (NCBI) | 143282 |
| RRID | AB_2879691 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAT2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FGFBP3 also named as C10orf13 or FGF binding protein 3 is 258 amino acid protein, which belongs to the fibroblast growth factor binding family. FGFBP3 is a secreted protein and may play a role in the regulation of gene transcription. The molecular weight of FGFBP3 is 29kDa, but our results detected the 60-65kDa protein by western blot. We infer that FGFBP3 may exist as dimer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FGFBP3 antibody 24725-1-AP | Download protocol |
| WB protocol for FGFBP3 antibody 24725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
