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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGF-8 Polyclonal antibody (20711-1-AP)

    WB analysis using 20711-1-AP (same clone as 20711-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20711-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FGF-8 Polyclonal antibody (20711-1-AP)

    WB analysis of HeLa using 20711-1-AP (same clone as 20711-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20711-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using FGF-8 Polyclonal antibody (20711-1-AP)

    WB analysis of L02 using 20711-1-AP (same clone as 20711-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20711-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using FGF-8 Polyclonal antibody (20711-1-AP)

    WB analysis of L02 using 20711-1-AP (same clone as 20711-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20711-1-PBS in a different storage buffer formulation.

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FGF-8 Polyclonal antibody, PBS Only

FGF-8 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20711-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

FGF8, Heparin-binding growth factor 8, HBGF-8, Fibroblast growth factor 8, FGF 8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20711-1-PBS targets FGF-8 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 8 (androgen-induced)
Calculated molecular weight 27 kDa
Observed molecular weight24 kDa
GenBank accession numberNM_006119
Gene Symbol FGF8
Gene ID (NCBI) 2253
RRIDAB_10733225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55075
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FGF8, also named as AIGF and HBGF-8, belongs to the heparin-binding growth factors family. FGF8 stimulates growth of the cells in an autocrine manner. It mediates hormonal action on the growth of cancer cells. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). The antibody is specific to FGF8.