×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FGF1 Polyclonal antibody (17400-1-AP)

    WB analysis using 17400-1-AP (same clone as 17400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17400-1-AP (FGF1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spinal cord tissue using FGF1 Polyclonal antibody (17400-1-AP)

    WB analysis of mouse spinal cord using 17400-1-AP (same clone as 17400-1-PBS)

    mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 17400-1-AP (FGF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FGF1 Polyclonal antibody (17400-1-AP)

    WB analysis using 17400-1-AP (same clone as 17400-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17400-1-AP (FGF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of human colon cancer using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of human colon cancer using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of human breast cancer using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of human breast cancer using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of human lung cancer using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of human lung cancer using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of mouse brain using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of mouse brain using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of rat brain using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of rat brain using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of rat kidney using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using FGF1 Polyclonal antibody (17400-1-AP)

    IHC staining of rat kidney using 17400-1-AP (same clone as 17400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17400-1-AP (FGF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FGF1 Polyclonal antibody (17400-1-AP)

    IF Staining of HepG2 using 17400-1-AP (same clone as 17400-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGF1 antibody (17400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 17400-1-PBS in a different storage buffer formulation.

FGF1 Polyclonal antibody, PBS Only

FGF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17400-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FGF-1, Endothelial cell growth factor, Fibroblast growth factor 1, HBGF-1, Heparin-binding growth factor 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17400-1-PBS targets FGF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11366

Product name: Recombinant human FGF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-155 aa of BC032697

Sequence: MAEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 1 (acidic)
Calculated molecular weight 155 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC032697
Gene Symbol FGF1
Gene ID (NCBI) 2246
RRIDAB_2103758
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP05230
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fibroblast growth factor 1 (FGF1), also known as acidic fibroblast growth factor (aFGF) and heparin-binding growth factor 1 (HBGF-1), is a 17-18 kDa signaling protein. As a member of the FGF family, it possesses broad mitogenic and cell survival activities, playing critical roles in embryonic development, tissue repair, angiogenesis, and organogenesis. FGF1 exerts its pleiotropic effects by binding to and activating its tyrosine kinase receptors, FGFR1 through FGFR4, in a process that is potentiated by cell-surface heparan sulfate proteoglycans. This activation initiates several downstream signaling cascades, including the MAPK/ERK and AKT pathways. Originally purified in 1975 and distinguished from its homolog FGF2 by its acidic isoelectric point, FGF1 is unique in that it lacks a classical signal peptide and is secreted via a non-classical, stress-induced pathway involving S100A13 and SYT1. Beyond its well-established extracellular functions in wound healing and as a potent inducer of DNA synthesis, FGF1 also possesses intracellular activities and can translocate to the nucleus to influence cell survival.

||
New chat

Able™

正在加载，请稍候...