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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGF-2 Polyclonal antibody (11234-1-AP)

    WB analysis using 11234-1-AP (same clone as 11234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11234-1-AP (FGF-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using FGF-2 Polyclonal antibody (11234-1-AP)

    FC experiment of HepG2 using 11234-1-AP (same clone as 11234-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FGF-2 (11234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using FGF-2 Polyclonal antibody (11234-1-AP)

    FC experiment of K-562 using 11234-1-AP (same clone as 11234-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human FGF-2 (11234-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11234-1-PBS in a different storage buffer formulation.

FGF-2 Polyclonal antibody, PBS Only

FGF-2 Polyclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 11234-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

FGF2, FGF 2, FGF B, FGF basic, FGFB

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11234-1-PBS targets FGF-2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 2 (basic)
Calculated molecular weight 21 kDa
Observed molecular weight31 kDa
GenBank accession numberNM_002006
Gene Symbol FGF2
Gene ID (NCBI) 2247
RRIDAB_2941976
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP09038
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

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