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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGD3 Polyclonal antibody (20347-1-AP)

    WB analysis using 20347-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using FGD3 Polyclonal antibody (20347-1-AP)

    WB analysis of HEK-293T using 20347-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FGD3 Polyclonal antibody (20347-1-AP)

    WB analysis of K-562 using 20347-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FGD3 Polyclonal antibody (20347-1-AP)

    IHC staining of human placenta using 20347-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20347-1-AP (FGD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using FGD3 Polyclonal antibody (20347-1-AP)

    IHC staining of human placenta using 20347-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20347-1-AP (FGD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FGD3 Polyclonal antibody (20347-1-AP)

    IF Staining of A549 using 20347-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 20347-1-AP (FGD3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

FGD3 Polyclonal antibody

FGD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20347-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Zinc finger FYVE domain-containing protein 5, ZFYVE5, FYVE, RhoGEF and PH domain-containing protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, K-562 cells, HEK-293T cells, HeLa cells, A549 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20347-1-AP targets FGD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14155

Product name: Recombinant human FGD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 570-725 aa of BC032429

Sequence: KAENSRQSRVCRDCFLTQPVAPESTEKTPTADPQPSLLCGPLRLSESGETWSEVWAAIPMSDPQVLHLQGGSQDGRLPRTIPLPSCKLSVPDPEERLDSGHVWKLQWAKQSWYLSASSAELQQQWLETLSTAAHGDTAQDSPGALQLQVPMGAAAP

相同性解析による交差性が予測される生物種
Full Name FYVE, RhoGEF and PH domain containing 3
Calculated molecular weight 725 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC032429
Gene Symbol FGD3
Gene ID (NCBI) 89846
RRIDAB_10693616
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JSP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FGD3 antibody 20347-1-APDownload protocol
IHC protocol for FGD3 antibody 20347-1-APDownload protocol
IF protocol for FGD3 antibody 20347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncogene

FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression.

Authors - Feng Guo
  • KD Validated
View Article