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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGD1 Polyclonal antibody (13141-1-AP)

    WB analysis using 13141-1-AP (same clone as 13141-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13141-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FGD1 Polyclonal antibody (13141-1-AP)

    WB analysis using 13141-1-AP (same clone as 13141-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13141-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SK-N-SH cells using FGD1 Polyclonal antibody (13141-1-AP)

    IP experiment of SK-N-SH using 13141-1-AP (same clone as 13141-1-PBS)

    IP result of anti-FGD1 (IP:13141-1-AP, 4ug; Detection:13141-1-AP 1:600) with SK-N-SH cells lysate 1160 ug. This data was developed using the same antibody clone with 13141-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FGD1 Polyclonal antibody (13141-1-AP)

    IHC staining of mouse kidney using 13141-1-AP (same clone as 13141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13141-1-AP (FGD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13141-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FGD1 Polyclonal antibody (13141-1-AP)

    IF Staining of U2OS using 13141-1-AP (same clone as 13141-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using FGD1 antibody (13141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13141-1-PBS in a different storage buffer formulation.

FGD1 Polyclonal antibody, PBS Only

FGD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13141-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13141-1-PBS targets FGD1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3861

Product name: Recombinant human FGD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 611-961 aa of BC034530

Sequence: YLILFNDRLLYCVPRLRLLGQKFSVRARIDVDGMELKESSNLNLPRTFLVSGKQRSLELQARTEEEKKDWVQAINSTLLKHEQTLETFKLLNSTNREDEDTPPNSPNVDLGKRAPTPIREKEVTMCMRCQEPFNSITKRRHHCKACGHVVCGKCSEFRARLVYDNNRSNRVCTDCYVALHGVPGSSPACSQHTPQRRRSILEKQASVAAENSVICSFLHYMEKGGKGWHKAWFVVPENEPLVLYIYGAPQDVKAQRSLPLIGFEVGPPEAGERPDRRHVFKITQSHLSWYFSPETEELQRRWMAVLGRAGRGDTFCPGPTLSEDREMEEAPVAALGATAEPPESPQTRDKT

相同性解析による交差性が予測される生物種
Full Name FYVE, RhoGEF and PH domain containing 1
Calculated molecular weight 901 aa, 91 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC034530
Gene Symbol FGD1
Gene ID (NCBI) 2245
RRIDAB_3085409
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP98174
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).

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