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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FFAR3 Monoclonal antibody (66811-1-Ig)

    WB analysis using 66811-1-Ig (same clone as 66811-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adipose tissue using FFAR3 Monoclonal antibody (66811-1-Ig)

    WB analysis of human adipose using 66811-1-Ig (same clone as 66811-1-PBS)

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FFAR3 Monoclonal antibody (66811-1-Ig)

    WB analysis of HepG2 using 66811-1-Ig (same clone as 66811-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FFAR3 Monoclonal antibody (66811-1-Ig)

    IHC staining of human liver cancer using 66811-1-Ig (same clone as 66811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66811-1-Ig (FFAR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FFAR3 Monoclonal antibody (66811-1-Ig)

    IF Staining of A549 using 66811-1-Ig (same clone as 66811-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 66811-1-Ig (FFAR3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

FFAR3 Monoclonal antibody, PBS Only

FFAR3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66811-1-PBS
Clone No.1D10B7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GPR41, 1D10B7, FFA3R, free fatty acid receptor 3, G protein coupled receptor 41

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66811-1-PBS targets FFAR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28025

Product name: Recombinant human FFAR3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 246-346 aa of BC035657

Sequence: VVGYICGESPAWRIYVTLLSTLNSCVDPFVYYFSSSGFQADFHELLRRLCGLWGQWQQESSMELKEQKGGEEQRADRPAERKTSEHSQGCGTGGQVACAES

相同性解析による交差性が予測される生物種
Full Name free fatty acid receptor 3
Calculated molecular weight 39 kDa
Observed molecular weight 40-48 kDa
GenBank accession numberBC035657
Gene Symbol FFAR3
Gene ID (NCBI) 2865
RRIDAB_2882154
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO14843
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FFAR3 (Free fatty acid receptors 3) also known as GPR41, with another GPR43 protein, both are Gai-coupled receptor activated by short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate (PMID:26870043). GPR41 protein is translated from the bicistronic mRNA encoding GPR40 and GPR41, where an internal ribosome entry site (IRES) is utilized for the GPR41 coding sequence downstream of GPR40 (PMID:22493486). GPR41 is expressed in adipose tissue, gut, and the peripheral nervous system, and it is involved in SCFA-dependent energy regulation(PMID: 24904531).

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