Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, HCT 116 cells, HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84544-1-RR targets FFAR2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | free fatty acid receptor 2 |
| Calculated molecular weight | 330 aa, 37 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC096198 |
| Gene Symbol | FFAR2 |
| Gene ID (NCBI) | 2867 |
| RRID | AB_3672049 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O15552 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FFAR2 antibody 84544-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |