Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| ELISA | See 1 publications below |
Product Information
21765-1-AP targets FFAR2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16367 Product name: Recombinant human FFAR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 270-330 aa of BC096198 Sequence: PLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE 相同性解析による交差性が予測される生物種 |
| Full Name | free fatty acid receptor 2 |
| Calculated molecular weight | 330 aa, 37 kDa |
| Observed molecular weight | 37-50 kDa |
| GenBank accession number | BC096198 |
| Gene Symbol | FFAR2 |
| Gene ID (NCBI) | 2867 |
| RRID | AB_3742056 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15552 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FFAR2 antibody 21765-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Nutr Food Res Monascus pilosus SWM-008-Fermented Red Mold Rice and Its Monascinol Ameliorate Non-Alcoholic Fatty Liver Disease via Activation of the AMPK-ATGL Pathway and Enrichment of Butyrate-Producing Bacteria. |