Validation Data Gallery
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Tested Applications
| Positive WB detected in | THP-1 cells, Karpas-299 cells, NR8383 cells, RAW 264.7 cells, TF-1 cells, HL-60 cells, U-937 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67524-1-Ig targets FES in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28108 Product name: Recombinant human FES protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 125-390 aa of BC035357 Sequence: QELTKTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEH 相同性解析による交差性が予測される生物種 |
| Full Name | feline sarcoma oncogene |
| Calculated molecular weight | 822 aa, 94 kDa |
| Observed molecular weight | 93 kDa, 79 kDa, 87 kDa, 85 kDa |
| GenBank accession number | BC035357 |
| Gene Symbol | FES |
| Gene ID (NCBI) | 2242 |
| RRID | AB_2882744 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07332 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FES antibody 67524-1-Ig | Download protocol |
| WB protocol for FES antibody 67524-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
