Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, THP-1 cells, U-937 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28194-1-AP targets FES in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28095 Product name: Recombinant human FES protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-390 aa of BC035357 Sequence: QELTKTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEH 相同性解析による交差性が予測される生物種 |
| Full Name | feline sarcoma oncogene |
| Calculated molecular weight | 822 aa, 94 kDa |
| Observed molecular weight | 93 kDa, 79 kDa, 87 kDa, 85 kDa |
| GenBank accession number | BC035357 |
| Gene Symbol | FES |
| Gene ID (NCBI) | 2242 |
| RRID | AB_2881086 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07332 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Feline sarcoma oncogene protein (FES) is a member of the FES/FES-related (FER) subfamily of non-receptor protein tyrosine kinases. FES was originally identified a retroviral oncogene in avian and feline retroviruses. Articles reported that FES plays a role in growth and differentiation of myeloid hematopoietic cells, vascular endothelial cells and neurons. And it also involved in the regulation of cytoskeletal rearrangement (PMID:15929003). Addition, a recent study suggests that FES may function both tumorigenic and tumor-suppressive effects (PMID: 28952025).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FES antibody 28194-1-AP | Download protocol |
| WB protocol for FES antibody 28194-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
